The Basic Principles Of flow cytometer

The Basic Principles Of flow cytometer

Blog Article

Facts-enriched characterization of mobile populations. Morphological information and facts from photos can incorporate into the richness of flow cytometry knowledge. For example, the figure exhibits an usually traditional apoptosis assay employing Annexin V and PI, adding mobile imaging to characterize cells in Every inhabitants to expose morphologically distinctive features. These insights could not are acquired from multiplex staining alone.

As samples are obtained over the Attune CytPix Flow Cytometer, the higher-speed brightfield digital camera captures and stores pictures of detected situations in a price as many as 6,000 pictures per next, depending upon the flow amount and impression sizing. For larger adaptability, Attune Cytometric Computer software permits you to adjust the graphic seize frequency as wanted and choose a certain gate for graphic capture, if necessary.

Combine acoustic concentrating know-how with substantial-parameter Assessment for versatile spectral unmixing and traditional workflows.

The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a large-speed brightfield digicam for simultaneous substantial throughput flow cytometry and large resolution brightfield imaging.

"A picture isn't really worthy of a thousand dots, nonetheless it definitely really helps to see photos. It is great to determine illustrations or photos that correlate with staining. For instance, AnnexinV-optimistic cells which can be morphologically various than balanced cells, or CD14+ monocytes which can be larger sized plus much more textured than CD3+/CD4+/CD14- lymphocytes.

Also, it's illuminating to see how many doublets slip through traditional singlet gates, and excellent to confirm the status of unpredicted double-constructive activities in my analysis."

Pair your Attune flow cytometer having an autosampler for enhanced workflow efficiency, and include robotic integration for max throughput.

Our researchers will layout panels for yourself as many times you require—free of charge, no product acquire wanted.

Early indications of this contamination had at first been dismissed as particles. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also enables you to use morphological attributes to find appealing subpopulations that could not be apparent from flow cytometry knowledge on your own.

Possibly most significantly, these events consist of cells of Plainly unique phenotypes which may lead to incorrect conclusions about double constructive gatherings (especially in rare populations).

AI-pushed automated image Examination While using the Attune Cytometric Program translates function characteristics into unique morphology parameters which might be coupled with normal here fluorescence and scatter parameters to expose really reproducible population improvements and exclusive subsets.

We previously demonstrated the strength of imaging Auto-T/Ramos cell interactions. Let’s check out just the populace of biggest desire, the double favourable gatherings, to learn more. We are able to now use extended morphological parameters (in this article, circularity and skewness of intensity) to more analyze the options of those populations and refine gating on these events, rising knowledge robustness.

Leverage the power of Highly developed know-how and innovation to speed up your discoveries. Have confidence in within the Attune Flow Cytometer loved ones to provide the reliability, pace, and accuracy that set your do the job aside.

No clean/no lyse leukocyte Evaluation with imaging. Cells have been obtained from 24-hour-outdated blood by dilution in one mM EDTA ( Qualified UX/UI allows buyers to coach AI types for expanded apps. (A) The “Practice Model” aspect supplies annotation equipment and authentic-time product schooling accuracy.

At the center of each Attune Flow Cytometer lies our innovative acoustic concentrating technologies, which makes use of sound waves to meticulously align cells inside the flow mobile. This innovative solution surpasses regular hydrodynamic procedures, resulting in more rapidly processing speeds, exceptional facts high-quality, and elevated sample throughput.

Because the mobile line experienced Earlier been analyzed about the Attune CytPix Flow Cytometer, the scientists went again to the images and were ready to document the microbial infection at the least five days before.

Agilent Benefit Guarantee guarantees you not less than ten years of instrument use with the day you buy. In any other case, We are going to credit you Together with the residual value of the process toward an upgraded model.

Right here, we present that by gating tactics using picture-primarily based quantitative parameters can distinguish interacting cells from coincident occasions to additional accurately evaluate interacting cells.